He was replaced at second base and in the batting order by Brandon Inge, who went 0-for-2 with a strikeout. Walker was 0-for-2 with a flyout and a strikeout before departing.
Walker was scheduled to be evaluated further Saturday afternoon. He entered the game hitting .246 with six home runs and 26 RBIs.
He went 1-for-4 with an RBI triple in Friday's series-opening 6-2 win against the Cubs.
Joey Nowak is a reporter for MLB.com. Follow him on Twitter at @joeynowak.& This story was not subject to the approval of Major League Baseball or its clubs.